PhD Scientific Days 2018

Budapest, April 19–20, 2018

Tissue-based in situ detection of the clarithromycin resistance for the personalized Helicobacter pylori eradication therapy

Kocsmár, Éva

Éva Kocsmár1, Mihály Kramer1, Gergely Röst2, György Miklós Buzás3, László Bene4, Attila Szijártó5, Ildikó Szirtes1, Zsófia Kramer1, Ildikó Kocsmár1, Petra Fadgyas-Freyler6, Gábor Urbán6, András Kiss1, Zsuzsa Schaff1, Gábor Lotz1

1 2nd Department of Pathology, Semmelweis University, Budapest, Hungary
2 Bolyai Institute, University of Szeged, Szeged, Hungary
3 Department of Gastroenterology, Ferencváros Health Centre, Budapest, Hungary
4 1st Department of Medicine, Péterfy Hospital, Budapest, Hungary
5 1st Department of Surgery, Semmelweis University, Budapest, Hungary
6 National Health Insurance Fund Administration, Budapest, Hungary

Language of the presentation


Text of the abstract

Introduction: Clarithromycin-resistance (Cla-res) is the leading cause of treatment failure of Helicobacter pylori (HP) infections.
Aims: Our aim was to examine the prevalence of clarithromycin-resistant HP-infection and its connection to gender and age in a monocentric cohort.
Method: 4744 HP-positive adults were examined (2708 females; 57.1%, 2036 males; 42.9%). HP-positive gastric mucosal tissue slides were investigated by a bacterial rRNA-targeted FISH-test (BactFISH Helicobacter Combi Kit) detecting the clarithromycin-sensitive and -resistant HP-bacteria. HP eradication-related and -independent antibiotic consumption anamneses of these patients were collected in cooperation with the Hungarian National Health Insurance Fund.
Results: Overall Cla-res rate was 17.2%. Females showed significantly (p<0.0001) higher Cla-res rate (19.8%) than males (13.7%). Low Cla-res prevalence (12.9%) was found in the age group 70+. Cla-res rate reached 20% in females aged under 70, while it was less than 15% in males except ages 30-39 (15.3%). Cla-res prevalence was significantly lower (5.52%) in macrolide-naive patients than in the macrolide-treated group (30.5%; p<0.001). No significant difference was found between macrolide-naive females and males (6.4% vs. 4.6%; p=0.057).
Conclusion: Gender and age distribution of clarithromycin-resistance should be considered for HP-eradication treatments. Our results suggest that higher prevalence of clarithromycin-resistance in females is related to increased exposition to macrolide antibiotics.

Data of the presenter

Egyedi azonosító: ÚNKP_17-3-II-SE-3
Doctoral School: Pathological Sciences
Program: Alterations of Cells, Fibres and Extracellular Matrix and Diagnostic Pathomorphological Studies in the Course of Heart and Vascular Diseases and in Certain Tumours. Experimental and Diagnostic Pathomorphological Studies
Supervisor: Gabor Lotz