Postovalova E.A.1,2, Makarova O.V.1,2
1 Research Institute of Human Morphology, Moscow, Russia
2 Lomonosov Moscow State University, Moscow, Russia
Ulcerative colitis (UC) is an inflammatory disease of the colon with an unknown etiology and relapsing course, it affects the mucosal layer of rectum and other sections of the colon. The quantitative evaluation of T-regulatory lymphocytes (T-reg) in mucosal biopsy of the colon of UC patients is proposed as one of diagnostic criteria by a number of authors.
Our study of morphological changes in the colon in conjunction with the systemic and local alterations of lymphocyte subpopulations in experimental chronic UC.
UC was induced by an oral administration of 1% solution of DSS in drinking water ad libitum for 4 days at first and then on 10–12 and 21–25 day followed by drinking of tap water. Lymphocytes of the lamina propria were isolated from colon specimens using modifications of previously described techniques. Analysis of peripheral blood and colon lymphocyte cells: CD3+CD4+ T-helper cells, CD3+CD8+ cytotoxic T-lymphocytes, CD3-CD19+ B-lymphocytes, CD4+CD25+Foxp3+ T-reg were determined by flow cytometry.
In chronic UC there were multiple small epithelium covered ulcers in the colon mucosa and pronounced features of remodeling – crypts were decreased in number, deformed, had enlarged lumens, there also were several crypt-abscesses. There were focal fibrosis of the mucosa and its inflammatory infiltration with lymphocytes, macrophages and plasmocytes. During chronic UC in the peripheral blood there was an increase in the absolute number of T-reg; in the colon there was an increase in the number of T-helpers, B-lymphocytes, T-reg.
In the colon there is distinct lymphoid-plasmocytic infiltration of the lamina propria mucosa, more pronounced in the distal part of the colon, there is an increase in number of T-helpers, B-lymphocytes. The number of T-reg increases in the peripheral blood and colon mucosa.
Doctoral School: Lomonosov Moscow State University
Program: Cell biology, cytology, histology
Supervisor: Makarova Olga
E-mail address: firstname.lastname@example.org