Dorottya Kardos1, István Hornyák1, Melinda Simon1Adél Hinsenkamp1, Bence Marshall1, Róbert Várdai2, Balázs Pinke3, László Mészáros3, Alfréd Kállay-Menyhárd4, Zsombor Lacza1
1Institute of Clinical Experimental Research, Semmelweis University, Budapest, Hungary
2Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
3 Department of Polymer Engineering, Budapest University of Technology and Economics, Budapest, Hungary
4Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Hungary
Platelet rich fibrin (PRF) membrane is a second-generation platelet concentrate, produced from autologous blood in a glass tube without any anticoagulant. It is a biocompatible 3D scaffold, containing fibrin clot, platelets, leukocytes and a high concentration of growth factors. It has been tested in numerous clinical situations usually in dental and maxillofacial applications.
We have produced a reproducible, sterile PRF membrane with constant thickness and diameter using the hypACT Inject Auto device. Our aim was to compare the traditional PRF membrane produced in a glasstube (GT-PRF) with the hypACT derived PRF membrane (HI-PRF) based on the following properties: mesenchymal stem cell (MSC) adhesion and proliferation capacity on the membranes, weight loss of the membranes during the culture period, tensile- and strain strength, and plasmin activity measurement in the PRF membranes. The examination of the surface and structure characteristics was assessed using electron microscopy and live-dead cell staining using confocal microscopy.
There were no significant differences in MSC adhesion, proliferation capacity and in the weight loss of the PRF membranes. There was also no significant difference in the tensile and strain strength values; however the typical stress-strain curves of the two types of membranes were different. The surface and structure of the membranes were similar, but in case of HI-PRF membrane platelets are located in the inside of the membrane, while in case of GT-PRF they are located mostly on the surface.
The biological properties of HI-PRF membrane are at least as good as GT-PRF. Furthermore, we can conclude, based on the stress-strain curve, that HI-PRF is homologous, and more suturable compared to GT-
-PRF, which could be an advantage in clinical use.
The hypACT device ensures an easy, fast, and sterile method to isolate PRF membrane from autologous blood. This membrane has the same excellent biological properties as the traditional one.
Basic Medicine, The Mechanisms of Normal and Pathologic Functions of the Circulatory System
Dr. Zsombor Lacza