Ivan Tsvetkov, RESEARCH INSTITUTE OF HUMAN MORPHOLOGY, Moscow, Russia
Evgeniy Tikhonov, RESEARCH INSTITUTE OF HUMAN MORPHOLOGY; LOMONOSOV MOSCOW STATE UNIVERSITY, Moscow, Russia
Vladimir Mkhitarov, RESEARCH INSTITUTE OF HUMAN MORPHOLOGY, Moscow, Russia
To characterize the morphofunctional changes of testis and the subpopulations of lymphocytes in male blood of mice C57BL/6 with supraphysiological testosterone concentration.
Methods of research
To achieve a supraphysiological level of testosterone, a mixture of testosterone esters Omnadren-250 was injected intramuscularly at the dose of 250 mg/kg. The duration of the experiment was 42 days.
Morphometric examination of the area and diameter of the testis was carried out.
The following antibodies were used for immunophenotypic analysis of lymphocytes: anti-Mouse CD3 (T-lymphocytes); anti-Mouse CD4 (T-helpers); anti-Mouse CD8 (cytotoxic T cells); anti-Mouse CD19 (B-lymphocytes); anti-Mouse\Rat Foxp3 (regulatory T cells). The absolute number of lymphocytes of different subpopulations was counted on the Cytomics FC 500 (Beckman Coulter, USA).
“Statistica 6” was used for statistical analysis. The data are presented in the form of a median and the lower and upper quartiles. Statistical processing of the obtained results was carried out by Mann-Whitney U test. Differences were considered significant at p≤0.05.
Compared to the control group in animals with a supraphysiological testosterone concentration, the diameter of the seminiferous tubules was significantly lower, reflecting a decrease in the area of the spermatogenic epithelium.
In the peripheral blood of mice with supraphysiological testosterone concentration, an increase in the relative amount of CD3+CD4+ and CD3+CD8+, as well as a decrease in the absolute number of CD19+ and CD4CD25Foxp3+ cells, were detected.
An increase in the number of forms with the pathology of the head in 2 times and a decrease in sperms motility by 20% were revealed. These changes are associated with a violation of growth and maturation of spermatozoa.
A prolonged supraphysiological testosterone concentration in C57BL/6 mice led to decreases of spermatogenesis with the development of oligo- and teratospermia.
Immune disorders are characterized by a shift in the balance of the subpopulation composition of lymphocytes with a decrease in the number of T-regulatory and B-lymphocytes.
Doctoral School: histology, cytology and cell biology
Program pathology and pathophysiology
Supervisor: Makarova O.V.