M. Aresti1,2, Z. Lohinai1, B. Zaks 2, Z. Tóth1, D. Steinberg 2
1 Department of Conservative Dentistry, Semmelweis University, Budapest, Hungary
2 Biofilm Research Laboratory, Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, Jerusalem, Israel
Introduction: Biofilms are present on the teeth as dental plaque, where they may cause tooth decay and gum disease. As many as 400 species of bacteria have been associated with dental plaques, one of the most common being Streptococcus mutans is associated with cariogenesis. The mechanical and chemical controls of biofilm growth is essential for the maintenance of good dental health.
Aims: The aim of this research was to examine the antibiofilm properties of non-toxic substances as sodium bicarbonate (NaHCO3) and newly developed highly pure chlorine dioxide (ClO2) on Streptococcus mutans.
Method: Streptococcus mutans biofilm formation and preformed biofilm destruction were examined after exposure of different concentrations of NaHCO3 and highly pure ClO2 (Solumium) with crystal violet staining (total biomass analysis), MTT assays (metabolic activity) and confocal laser scanning microscopy (ratio of dead/live bacteria in different biofilm depth) and analysed by ANOVA.
Results: Our results revealed significant inhibition of Streptococcus mutans biofilm formation with NaHCO3 or more powerful with ClO2 in comparison with biofilm formation alone. NaHCO3 displayed much less demolition on preformed biofilm than ClO2. These observations mirrored the differences found in investigation of their minimal inhibitory concentration.
Conclusion: Our data demonstrate that both NaHCO3 and highly pure ClO2 are powerful inhibitors of biofilm development, but show less effects on the biofilm destruction. Clinical application of these agents is suggested as effective non-toxic biocides for the prevention of dental biofilm formation and therefore the consequent tooth decay.
Doctoral school: Clinical Medicine
Program: Antibacterial agents against dental biofilm
Supervisor: Dr. Zsolt Lohinai
E-mail address: email@example.com