PhD Scientific Days 2018

Budapest, April 19–20, 2018

TGFβ1 GENE POLIMORPHISM INFLUENCE THE OUTCOME OF HEMATOPOIETIC STEM CELL TRANSPLANTATION

Kövy, Petra

1 Petra Kövy, Laboratory of Molecular Genetics, Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
2 Nóra Meggyesi, Laboratory of Molecular Genetics, Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
Lívia Varga, School of PhD Studies, Semmelweis University, Budapest
Katalin Balassa, School of PhD Studies, Semmelweis University, Budapest
András Bors, Laboratory of Molecular Genetics, Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
Árpád Batai, Department of Haematology and Stem Cell Transplantation,Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
Éva Torbágyi, Department of Haematology and Stem Cell Transplantation,Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
László Gopcsa, Department of Haematology and Stem Cell Transplantation,Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
Lilla Lengyel, Department of Haematology and Stem Cell Transplantation,Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
Anikó Barta,Department of Haematology and Stem Cell Transplantation,Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
Attila Tordai, Department of Pathophysiology, Semmelweis University, Budapest
Tamás Masszi, 3rd Department of Internal Medicine, Semmelweis University, Budapest
Péter Reményi, Department of Haematology and Stem Cell Transplantation,Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest
Hajnalka Andrikovics, Laboratory of Molecular Genetics, Central Hospital of Southern Pest National Institute of Hematology and Infectious Diseases, Budapest

Language of the presentation

Hungarian

Text of the abstract

INTRODUCTION Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a curative therapeutic option for malignant hematopoietic diseases with a significant transplant-related mortality. Acute graft versus host disease (aGvHD) is a frequent, life threatening complication of allo-HSCT. Cytokines including transforming growth factor β1 (TGFβ1) play pivotal role in the development of aGvHD.
AIMS The aim of this study was to investigate the role of TGFβ1 -1347C>T polymorphism (rs1800469) in the outcome of HSCT.
METHOD We examined the correlation of recipient and donor TGFβ1 -1347C>T status and allo-HSCT outcome in a cohort of 424 adult patients who underwent first allo-HSCT between 2007 and 2013. 217 patients were transplanted with their siblings, 202 patients with matched unrelated donors (MUD) and 5 patients with other relatives. TGFβ1 rs1800469 was analysed from genomic DNA with hybridisation probes method on a LightCycler instrument.
RESULTS We did not find any association between recipient TGFβ1 -1347C>T polymorphism and HSCT outcome. In case of myeloablative conditioning therapy the donor TGFβ1 genotype correlated with overall survival (60 month) CC: 61,5±4,8%; CT: 46,0±4,7%; TT: 35,6±9,3% (p=0.034), which was independent of age and donor type (sibling/MUD) multivariate analizis. In the effect of the observed difference not only relapse (CC: 18%, CT: 28%, TT: 18%; p=0.18), but also acut graft versus host disease grade III-IV had increased frequency [CC: 11% (12/109); CT: 16% (20/122); TT: 24% (8/33), p=0.056 trend].
CONCLUSION Our results suggest that donor TGFβ1 -1347C>T polymorphism may influence the developement of aGvHD and the overall survival in patients with myeloablative conditioning.

Data of the presenter

Doctoral School: Clinical Medicine
Program: Clinical Haematology
Supervisor: Hajnalka Andrikovics (Program Supervisor: Tamás Masszi)
E-mail: andrikovics.hajnalka@dpckorhaz.hu