Kovács ÁF1, Héjja H2, Fekete N1, Alasztics B2, Joó G2, Szabó-Taylor K1, Buzás E1, Rigó J2, Pállinger É1
1Dept. of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
21st Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary
Introduction: Preeclampsia is a multi-system disorder without reliable biomarkers and causative treatment. Meanwhile, it is one of the main risk factors of adverse pregnancy outcome. Its pathogenesis is associated with systemic inflammation and imbalanced redox homeostasis.
Aim: Detection of multifaceted oxidative stress markers from blood plasma, blood borne extracellular vesicles (EVs) and peripheral blood mononuclear cells (PBMCs) for complex monitoring of the in vivo redox status in pregnancies complicated with preeclampsia.
Methods: In vivo oxidative damage of blood plasma proteins (total thiol level, advanced oxidation protein products, oxidized LDL), exofacial thiols of PBMCs, circulating EVs, thioredoxin1 (TRX1) and peroxiredoxin1 (PRDX1) regulatory enzymes of redox homeostasis were determined in preeclampsia (n=15). Third trimester healthy pregnant women were accepted as control group.
Results: Preeclampsia is associated with significant elevation of plasma total thiol concentration (p=0.002), increased exofacial thiol content on circulating PBMCs (p<0.0001), and lower exofacial thiol on EVs (p=0.0002). The expression level of the regulatory enzyme peroxiredoxin-1 was also significantly higher (p <0.05) on the surface of lymphocytes, although neither the plasma concentration of soluble PRDX1 nor the EV-surface PRDX1 differed. Exofacial TRX1 expression of PBMC was also significantly higher (p<0.0001) in preeclamptic patients.
Conclusion: Complex methods are required for the characterization of in vivo redox homeostasis in preeclampsia. Simultaneous detection of oxido-reductive state at cellular and vesicular levels is a more sensitive approach for the characterization of pregnancy induced hypertensive complications than previously used biomarkers.
Doctoral School: Molecular Medicine
Program: Basis of Human Molecular Genetics and Gene Diagnostics
Supervisor: Éva Pállinger
E-mail address: firstname.lastname@example.org