1 Author Bernadett Martinecz
Nikolett Lénárt, Eszter Császár, Csaba Cserép, Barbara Orsolits
2 Author Adam Denes
Laboratory of Neuroimmunology IEM HAS
Aim: Inflammation plays a crucial role in diseases of the central nervous system. Inflammatory actions of microglia, the main inflammatory cells in the brain have been widely studied, but their functional contribution to brain injury is controversial. Previously we have shown that microglia contact injured neurons in an activity-dependent manner and the lack of microglia dysregulated neuronal network activity in the injured brain. However the exact details of microglia-neuron communication remained unexplored.
Methods: To investigate the molecular mechanisms regulating a novel form of microglia-neuron contact we have recently discovered, we aimed to develop in vivo imaging protocols combined with optogenetic or chemogenetic activation of neurons.
Results: Since our results show that purinergic signaling via the P2Y12 receptor is essential for microglia-neuron interactions, we have also tested the effects of P2Y12 receptor deficiency on microglial process motility and microglia-neuron interactions in vivo. We have also used high resolution microscopy to investigate the molecular anatomy of microglia-neuron contacts.
Conclusion: Collectively, our results reveal novel molecular pathways that contribute to the regulation of neuronal activity by microglia, which could be important to understand the pathophysiology of common brain diseases.
Doctoral School: Neurosciences (Szentagothai Janos)
Program : Neuromorphology and Cell Biology
Supervisor: Adam Denes