Arash Mirzahosseini, Semmelweis University Department of Pharmaceutical Chemistry
Béla Noszál, Semmelweis University Department of Pharmaceutical Chemistry
Nuclear magnetic resonance (NMR) spectroscopy and magnetic resonance imaging (MRI) are prime techniques in chemical research and medical diagnostics, providing indispensable molecular and morphologic information. In vivo magnetic resonance spectroscopy (MRS), the emerging cousin of the above two methods that broadcasts in vivo, molecular, real-time information in a noninvasive manner. Currently, the optimum performance of MRS studies can be achieved if in vitro plus in vivo magnetic resonance knowledge and experience are brought into method developments on high field instruments. It has been shown that the pH and redox status in the various regions of the brain are related to particular CNS diseases.
We therefore sought to determined the physico-chemical and spectroscopic parameters of important brain metabolites (phosphate metabolites, homocarnosine, lipoic acid) with high resolution biomimetic NMR (14.1 tesla). In perspective, these data will be validated in MRS phantoms and small animals, and finally applied to human studies, by means of synergic spectroscopies, observing 31P, 1H and 13C nuclei.
The 31P and 1H NMR spectra were recorded with a Varian 14.1 tesla NMR spectrometer. The pH values were determined with in situ NMR pH indicators and glass electrode calibrated with PBS buffers.
The acid-base, redox, and lipophilicity properties of the synthesized brain metabolites were characterized by NMR-based titrimetry, pH-potentiometry, UV-titrimetry, and quantitative NMR for redox chemistry.
Based on the physico-chemical properties of brain metabolites a more accurate in vivo pH and redox potential determining method can be developed based on magnetic resonance spectroscopy.
Post-doc: Department of Pharmaceutical Chemistry