Kosyreva A.M.1, Dzhalilova D.Sh.1, Diatroptov M.E.1, Tsvetkov I.S.1, Makarova O.V.1
1 Science Research Institute of Human Morphology, Department of Immunology of Inflammation, Moscow, Russia
Introduction. In the development of SIRS, hypoxia resulting from microcirculatory disorders plays a key role. Acute inflammation in females develops less frequently than in males, which may be due to the higher resistance of females to hypoxia.
Aim. To investigate the differences of LPS-induced morphological changes in the thymus and subpopulation composition of peripheral blood lymphocytes in female Wistar rats with high (HR) and low (LR) resistance to hypoxia.
Methods. Based on their gasping time in a hypobaric chamber, female Wistar rats were divided to HR (>240 seconds) and LR (<80 seconds). SIRS was modeled by intraperitoneal injection of 1.5 mg/kg LPS E.coli O26: B6. The volume fraction of the thymic cortex, the area of necrosis in the liver, and the number of neutrophils in the lungs were measured. A flow cytometry was used to investigating of the relative and absolute number of T- and B-lymphocytes in the blood and the number of apoptotic dying Annexin+ thymic cell. Statistical analysis was carried out using the criteria of Kruskal–Wallis and Dann.
Results. In both HR and LR females, the development of SIRS was accompanied by an involution of the thymus, apoptosis of the thymic cells, and an increase in the absolute number of NK. However, in HR females, inflammatory changes in target-organs were minimal, the involution of the thymus and thymocytes apoptosis were less pronounced, the number of T-helpers and Treg in the blood were decreased. In LR females, more pronounced inflammation was accompanied by increase of Treg and T-helpers.
Conclusion. LPS-induced immune response in females depends on individual resistance to hypoxia. The more severe inflammation in LR females is accompanied by pronounced apoptosis of thymocytes and an increase of T-helpers and Treg in the blood, which is an unfavorable prognostic factor.
Doctoral School: Biomedical science
Program: Cell biology, cytology and hystology
Supervisor: Olga V. Makarova
E-mail adrdress: email@example.com