Dóra Noémi Barczikai (1), Anikó Tóth(1), Viktória Kacsari(1), Gabriella Csík(2), Angéla Jedlovszky-Hajdú(1)
(1): Semmelweis University, Department of Biophysics and Radiation Biology, Nanochemistry Research Group, 1089, Budapest, Nagyvarad sq. 4, Hungary
(2): Semmelweis University, Department of Biophysics and Radiation Biology, Budapest, Hungary
Poly(amino acids) and derivatives are a promising new class of biomaterials but one of their drawbacks is their relatively difficult synthesis and functionalization. Polysuccinimide (PSI) can easily react with nucleophilic compounds therefore resulting in water soluble poly(aspartic acid) (PASP) and its derivatives.
Electrospinning is an efficient and inexpensive method for polymer processing,that allows the formation of nonwoven fibrous polysuccinimide meshes which could be used as wound dressings. Incorporation of silver nanoparticles (AgNPs) could provide antibacterial properties to the fibrous system. Minimizing pain could also be achieved by encapsulation of small-molecule drugs such as paracetamol.
The aim of our research was developing a one-pot method for the synthesis of AgNPs in the presence of a biocompatible poly(amino acid) derivative, then creating a novel antibacterial wound dressing system and finally investigating its antimicrobial properties, release profile and hydrolitical stability.
AgNPs were synthesised by chemical reduction method in the presence of PSI then the resulting nanoparticles were characterized with DLS and Uv-vis spectroscopy. Fibrous meshes were prepared using an electrospinning device and the surface structure was examined by Scanning Electron Microscopy. For the evaluation of antibacterial properties disc diffusion tests were carried out. Release kinetics of paracetamol was studied using UV-Vis spectroscopy.
By changing the synthesis and electrospinning parameters, an optimization procedure was carried out and drug-loaded electrospun PSI fibers containing AgNPs were successfully prepared. Antibacterial studies confirmed that silver-content hinders the colonisation of the meshes by E. coli. Based on the release kinetics studies, the synthesized antibacterial meshes proved to be suitable for prolonged drug-release.
AgNP and drug-containing PSI meshes were successfully prepared. Evaluation of antibacterial properties and release kinetics suggest that these matrices could be promising candidates for wound dressing as they can inhibit the colonisation of the wound site by pathogenic bacteria and ease the pain of the injured area.
Doctoral School: Basic and Translational Medicine
Program: Cellular and molecular biophysic
Supervisor: Angéla Jedlovszky-Hajdú, Dr.