Rita Varga1, Kristóf Molnár1, Máté Mihály Gulácsi1, Krisztina Nagy2, Angéla Jedlovszky-Hajdú1
1 Departement of Biophysics and Radiation Biology, Laboratory of Nanochemistry, Semmelweis University, 1089, Budapest Nagyvárad tér 4.
2 Department of Oral Biology, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary
Tissue engineering is one of the most intensively studied fields of medicine. A new direction in medicine is the regeneration of damaged tissue by culturing cells on a host artificial matrix and implanting it back to the damaged area to induce regeneration, trying to restore their original structure and function. For this, there is a constant need for new materials, where cells can adhere and multiply.
Hydrogel fibrous meshes prepared by electrospinning would be ideal materials mimicking the native soft tissue with their high water content and fibrous structure. Poly(amino acid) based hydrogels are emerging materials aiming for bio-medical applications. Their peptide like structure can be easily degraded by enzymes thus their biodegradability is ensured.
We optimized the produce of the polysuccinimide (anhydride of poly(aspartic acid)) fibers by electrospinning. In our research we made cross-linked poly(aspartic acid) polymers with hydrolysis, which contain disulphide bonds and 1,4-diaminobutane as cross linkers. FTIR microscopy was used to confirm the chemical structure of the polymers. With SEM we assessed the fibre diameter distribution. We tested the ability of cell to multiply on these artificial tissues on MG63 human osteosarcoma, for 24 and 72 hours with WST 1 reagent.
With the aid of previous studies of the reseach group, crosslinked poly(aspartic acid) nanofibers as artifical tissues were prepared. Via crosslinkers we prevented the hydrogel from dissolving in water. During the test of cell viability we found that, in presence of these membranes the cells can convert the monitoring reagent (WST-1). Hence we have observed, that the artificial tissues are non-toxic for the cells, but we need more experiments to prove, that they are really suitable as scaffolds.
The use of this type of biodegradable implant can be a huge step forward towards tissue regeneration, because the poly(aspartic acid) mesh has desirable chemical, mechanical and biological properties.
Doctoral School: Basic and Translational Medicine
Program: Cellular and molecular biophysics
Supervisor: Angéla Jedlovszky-Hajdú
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