PhD Scientific Days

Poster Presentation: Oncology

P32: Imaging of hepatic function following portal vein ligation in rat – The Old, the New and the Shiny

Előadó neve

Kovács, Tibor

Előadó munkahelye

1st Department of Surgery, Semmelweis University

Előadó telefonszáma

+36303124422; +36208252162

Előadó e-mail címe

ktib1991@gmail.com

Az előadás címe

Imaging of hepatic function following portal vein ligation in rat – The Old, the New and the Shiny

Szerző(k) neve és munkahelye

Tibor Kovács1, András Fülöp1, András Budai1, Dóra Tihanyi1, Kristóf Illés1, Dániel Sándor Veres2, Ildikó Horváth2, Krisztián Szigeti2, Domokos Máthé2,3, Attila Szijártó1
1 Hepato-Pancreatico-Biliary Surgery Research Center Hungary, 1st Department of Surgery, Semmelweis University, Budapest, Hungary
2 Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3 CROmed Translational Research Centers, Budapest, Hungary

Szekció

Poster Presentation: Oncology

Data of the presenter

Doctoral School of Clinical Medicine
Program: Hepatobiliary diseases - experimental and clinical aspects
Supervisor: Attila Szijártó
E-mail: ktib1991@gmail.com; kovacs.tibor1@med.semmelweis-univ.hu

Text of the abstract

Introduction. Evasion of posthepatectomy liver failure during extensive liver resections is clinically frequently managed by portal vein ligation (PVL), generating the atrophy- and hypertrophy of liver lobes with ligated and intact portal inflow, respectively. Morphological alterations are well-described, while functional alterations remain vague.
Aims. Our goal was the multimethodical morpho-functional assessment of post-PVL liver function in rat.
Materials and methods. PVL affecting ~80% liver parenchyma was performed on male Wistar rats (Σn=42). Liver morphology and global hepatic function were determined preoperatively, as well as 24h/48h/72h/168h/336h following PVL with serial MRI-volumetry, indocyanine-green clearence (ICG) (PDR, RT15) and 99mTc-mebrofenin hepatobiliary scintigraphy (HBS) (uptake-B1/2, excretion-DSTART) (n=12). Regional hepatic function was evaluated by HBS lobar peak count ratios, as well as confocal laser endomicroscopy (CLE) (T1/2, Tmax) in matching time points (n=30).
Results. MRI-volumetry confirmed the atrophy of ligated lobes (LL) and the hypertrophy of non-ligated lobes (NLL) (p<0.0001). Global hepatic function, as well as global hepatic uptake and -excretion were depressed between 24-72h according to ICG (p<0.01) and HBS (p<0.05). HBS also exhibited temporary decrease of bilateral regional hepatic function, followed by NLL functional re-estabilishment ultimately exceeding preoperative values at 336h; while LL function was permanently diminished (p<0.05). CLE confirmed the deterioration of NLL-specific uptake- and excretion at 72h, followed by normalisation towards 336h (p<0.05).
Conclusion. Beyond the atrophy-hypertrophy complex of the LL and NLL, return of transitionally depressed global hepatic function may be attributed to a solid functional gain of the NLL. CLE could be a capable method for selective liver functional measurement.

Funding: OTKA- K115607.

Azonosító

P32

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1031

PhD Scientific Days 2017

Budapest, 11-12 April 2017