PhD Scientific Days 2025

Poster Session III. - C: Molecular Medicine

Morphologic changes of lung, kidneys, thymus and spleen after acute hypoxic exposure in animals with different tolerance to hypoxia

Name of the presenter

Tsvetkov Ivan

Institute/workplace of the presenter

Petrovsky National Research Centre of Surgery

Authors

Tsvetkov Ivan¹, Silina Maria¹, Dzhalilova Dzhuliia¹, Mayak Margarita¹

1: Petrovsky National Research Centre of Surgery

Text of the abstract

Organisms differ according to hypoxia tolerance, which can determine the inflammatory and tumor diseases course. One of the approaches focused at increasing tolerance to infections is the use of the acute hypoxic exposure (AHE) of different levels. However, no data on such effect contribution on the morphofunctional state of internal organs depending on the initial organism hypoxia tolerance were stated in literature.
The aim of this study was to analyze morphological changes in lungs, kidneys, thymus and spleen after AHE in tolerant and susceptible to hypoxia animals.
The study was performed on adult male Wistar rats aged 2-3 months (n=60). One month before modeling of SHE, 1 ml of peripheral blood was obtained from the caudal vein and leukocytes were isolated. SHE was modeled once in a ventilated decompression chamber at an «altitude» of 11500 m. According to the «gasping time» (GT) – time to the lateral position achieved, animals were divided into tolerant (TH, n=13, GT>240 s) and susceptible (SH, n=12, GT<80 s) to hypoxia. One month later, AHE was performed (7000 m, 1 h) in a ventilated decompression chamber, after which the animals were euthanized from the experiment. Morphological and morphometric studies of lungs, kidneys, thymus and spleen were performed. The differences significance was determined using nonparametric criteria and was considered statistically significant at p<0.05.
Perivascular and intraalveolar oedema was revealed in the lungs of both TH and SH rats after AHE, but only TH rats demonstrated an increase in the neutrophils number in the interalveolar septa. Only in SH rats after AHE in kidneys dystrophy of convoluted tubules epithelium was revealed. After AHE in the thymus of SH, thymocytes death was revealed in the thymus, whereas in TH – hyperplasia of the cortex.
AHE was revealed to lead to morphological changes in organs sensitive to hypoxia – lungs and kidneys, as well as in organs of the immune system – thymus and spleen, which were more pronounced in SH animals.
This work was financially supported by the Russian Science Foundation (№25-15-00117).