PhD Scientific Days 2019

Budapest, April 25–26, 2019

Sex differences in the severity of glomerulosclerosis in Venus transgenic rabbits

Lipták, Nándor

Lipták, Nándor PhD; Bősze, Zsuzsanna PhD; Hiripi, László PhD
NARIC Agricultural Biotechnology Institute, Animal Biotechnology Department

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Several transgenic (TG) and non-TG rodent models were developed for investigating human focal segmental glomerulosclerosis (FSGS), but only a few laboratory rabbit models were created and characterized regarding this disease. In line with the data about glomerulosclerosis in CAGGS-EGFP mice, the excessive expression of the fluorophore in the glomeruli was associated with FSGS in Venus TG bucks. The vast majority of clinical investigations claimed that men have a worse outcome than women in the severity and progression of FSGS.
In the present study, the severity of FSGS was examined in Venus TG rabbits generated by Sleeping Beauty transposition and age-matched wild type (WT) controls of both sexes.
Urinalysis, hematology, serum biochemistry and renal cortex histology (H&E and PAS stainings on paraffin-embedded sections) were performed. Venus protein fluorescence was detected by confocal microscopy.
Hematology and selected serum biochemistry parameters (creatinine, albumin, cholesterol and triglycerides) were within normal reference ranges in all groups. The renal cortex sections of Venus TG bucks and does exhibited similar Venus fluorescence pattern. Urinalysis revealed mild proteinuria in Venus TG bucks, but not in Venus TG does and WT rabbits. The early sings of FSGS (glomerulomegaly and sclerotic glomeruli) were observed in Venus TG rabbits from both sexes, especially in bucks, but not in WT rabbits.
Similarly to human patients suffering from FSGS, male predominance was also observed in the severity of glomerulosclerosis in Venus TG rabbits.
This work was supported by the National Research, Development and Innovation Office (NKFIH) grant no. 108921, NVKP_16-1-2016-0039 to B.Z. and 120870 to L.N.

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