Krisztina Tóth1,2, Dávid Juriga1, Angéla Jedlovszky-Hajdú1, Miklós Zrínyi1, Gábor Varga2, Krisztina Nagy2
1 Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Budapest
2 Department of Oral Biology, Semmelweis University, Budapest
Introduction: Biopolymers are suitable materials for preparing drug delivery systems. Poly(aminoacid)s with high degree of polymerization have outstanding degradation property at physiological conditions. These polymers such as poly(succinimide) are chemically similar to the natural proteins and provide prolonged drug release with reduced side effects. Applying the electrospinning technique, fibrous polymer systems can produced with a diameter in the nano- or micrometer range. These meshes having large specific surface and porosity can increase the dissolution kinetics and absorption of polymer-drug conjugates. Due to these favorable properties better therapeutic effect can be achieved.
Aims: The aim of this work was to prepare biocompatible and biodegradable polymer-drug conjugates using poly(aspartamid) based nanofibers and to investigate the incidental citotoxicity of these systems.
Method: For preparating polymer-drug conjugates, poly(succinimide) was used, which is an anhydrous form of poly(aspartic acid). In this study, poly(succinimide) was conjugated with dopamine and formulated by electrospinning. The dopamine-polymer conjugates were characterized by scanning electon microscopy, atomic force microscopy and two-photon microscopy. The kinetics of dopamine release and the solubility were monitored by UV-VIS spectroscopy. The biocompatibility was examined by culturing of human periodontal ligament stem cells in the presence of these polymer-dopamine conjugates. The cell viability was assessed by WST-1 proliferation reagent and cell morphology was observed by phase-contrast microscopy and two-photon microscopy as well.
Results: Dopamine containing nanofibrous poly(aspartamide) drug conjugates were preapared with prolonged drug release. The cell viability results show the biocompatibility of these conjugates. Applying these dopamine-polymer conjugates, the concentration of dopamine can be higher than in case of treatment with free dopamine.
Conclusion: Due to biocompatibility and biodegradability these systems cause reduced side effects which is promising features for the therapy in the future.
Acknowledgements: This research was supported by UNKP-17-3-III-SE-17, EFOP-3.6.2-06-2017-00006, OTKA-NKTH CK-80928, OTKA-K115259, NKFI FK 124147.
Doctoral School: Clinical Medicine
Program: Dental Research
Supervisor: Gábor Varga
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