TT_I_P: Theoretical and Translational Medicine I. Posters
Zsófia Onódi1,2, Tamás Visnovitz3, Bernadett Kiss1,4, Anna Koncz3, Bence Ágg1,4,7, Barnabás Váradi1, Viktória É. Tóth1,2, Dorottya Gergő1,2, András Makkos1,4, Csilla Pelyhe1, Nóra Varga5, Dóra Reé5, Ágota Apáti5, Przemyslaw Leszek6, Péter Ferdinandy1,4,7, Edit I. Buzás3, Anikó Görbe1,4,7, Zoltán Giricz1,4,7, Zoltán V. Varga1,2
1 Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary
2 HCEMM-SU Cardiometabolic Immunology Research Group, Budapest, Hungary
3 Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
4 MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary
5 Research Centre for Natural Sciences, Institute of Enzymology, Budapest, Hungary.
6 Department of Heart Failure and Transplantology, Cardinal Stefan Wyszyński Institute of Cardiology, Warszawa, Poland
7 Pharmahungary Group, Szeged, Hungary
Background: Cardiac cell lines and primary cell cultures are widely used to model various cardiovascular diseases in vitro. Despite the increasing number of publications using these models, detailed comparative characterization of these cell lines has not been performed, therefore, limitations of these cell lines are still undetermined. The aim of our study was to compare the most commonly used cardiac cell lines to primary cultures and to mature cardiac tissues from the corresponding species by transcriptomic analysis and morphological characterization.
Methods: H9C2 (rat), AC16 (human) and HL-1 (mouse) cardiac cell lines were differentiated towards a phenotype more resembling cardiomyocytes, by methods most widely used in the literature, and cells were harvested at stages of proliferation and differentiation. Whole left ventricular tissue from each corresponding species, neonatal primary cardiac myocytes isolated from mice and rats, or human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) were applied as references for comparison. Transcriptome analysis and immunocytochemical detection of cardiac structural proteins were performed on all cell models. Results: RNA expression of cardiac markers (e.g. Tnnt2, Ryr2, Tnni3) was markedly lower in cell lines compared to primary cells or hiPSC-CM and adult tissue controls. Differentiation procedures induced a significant increase in cardiac- and decrease in embryonic markers in AC16 and H9C2 lines; however, the overall expression pattern of investigated genes in all cell lines showed significant differences in comparison to corresponding myocardium or primary cultures. Immunocytochemistry confirmed low expressions of structural protein alpha-actinin, troponin I and caveolin 3 in cell lines.
Conclusion: Expression patterns of cardiomyocyte markers and mRNA profile indicates low-to-moderate similarity of cell lines to primary cells/cardiac tissues regardless the differentiation protocol used. These limitations should be taken into account while choosing cells as in vitro platforms to model cardiomyocytes and cardiovascular diseases.
Semmelweis University, Doctoral School of Pharmaceutical Sciences