TT_I_L: Theoretical and Translational Medicine I. Lectures
Barbara Barath 1, Adam Varga 1, Adam Attila Matrai 1, Krisztina Deak-Pocsai 2, Norbert Nemeth 1, Adam Deak 1
1 Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen
2 Department of Physiology, Faculty of Medicine, University of Debrecen
INTRODUCTION: Polycystic ovary syndrome (PCOS) is one of the most common endocrinological diseases in women. Although the risk of cardiovascular diseases is high in PCOS, the number of scientific publications describing hemorheological changes is not significant.
AIMS: The aim of our research was to perform a comprehensive hematological and microrheological study on an experimentally induced PCOS in rats.
METHODS: Wistar rats were divided into two groups: the control group (n=9) and the PCOS group (n=9, animals received single-dose estradiol valerate subcutaneously). Measurements were carried out before treatment and then monthly for four months thereafter. Bodyweight, blood glucose level (AccuCheck Active), hematological parameters (Sysmex K-4500), erythrocyte deformability, membrane stability (LoRRca ektacytometer), and aggregation (Myrenne MA-1 aggregometer) were measured. The staging of the estrous cycle was done by examination of the vaginal smear. Histological examination of the ovary was performed at the end of the experiment.
RESULTS: A significant decrease vs baseline and vs Control group in red blood cell count (4 months vs base, p=0.031), hemoglobin (2 months vs base, p<0.001; 2 months vs control, p<0.001), and hematocrit (4 months vs base, p=0.005; 4 months vs control, p=0.049) in PCOS group were detected. Maximal elongation index showed a significant increase in the PCOS group (3 months vs base, p<0.001; 4 months vs base, p=0.05). In the PCOS group a significant increase was observed in several aggregation index parameters (M5s 4 months vs base, p<0.001; 4 months vs control, p<0.001; M1 5s 4 months vs base, p<0.001; 4 months vs control, p=0.014). The blood glucose level and the bodyweight were significantly elevated (4 months vs base, p=0.021; 4 months vs base p=0.003) in the PCOS group. The histological examination confirmed the developed PCOS.
CONCLUSION: PCOS-specific alterations were detected in the metabolic parameters, in the estrous cycle, and in histological features. The administration of estradiol valerate caused significant changes during the examined period in hematological and hemorheological parameters. Our results draw attention to the possible usefulness of micro-rheological investigations in further studies on PCOS.
FUNDING: The study was partially supported by the EFOP-3.6.3-VEKOP-16-2017-00009.
Debrecen University Faculty of Medicine, Doctoral School of Clinical Medicine