CL_VI_L: Clinical Medicine VI. Lectures
Csenge Pajtók1; Apor Veres-Székely1,2; Róbert Agócs1; Beáta Szebeni1,2; Péter Dobosy3; István Németh4; Zoltán Veréb4; Lajos Kemény4; Attila J. Szabó1,2; Ádám Vannay2; Tivadar Tulassay1,2; Domonkos Pap1,2
1 1st Department of Paediatrics, Semmelweis University, Budapest
2 ELKH-SE Pediatrics and Nephrology Research Group, Budapest
3 ÖK Danube Research Institute, Centre for Ecological Research, Budapest
4 Department of Dermatology and Allergology, Faculty of Medicine, University of Szeged
Introduction: Recent animal studies, as well as quantitative sodium MRI observations on humans demonstrated that remarkable amounts of sodium can be stored in the skin. It is also known that excess sodium in the tissues leads to inflammation in various organs, but its role in dermal pathophysiology has not been elucidated.
Aims: Our aim was to study the effect of dietary salt loading on inflammatory process and related extracellular matrix (ECM) remodeling in the skin.
Methods: To investigate the effect of high salt consumption on inflammation and ECM production in the skin mice were kept on normal (NSD) or high salt (HSD) diet and then dermatitis was induced with imiquimod (IMQ) treatment. The effect of high salt concentration on dermal fibroblasts (DF) and peripheral blood mononuclear cells (PBMC) was also investigated in vitro.
Results: The HSD resulted in increased sodium content in the skin of mice. Inflammatory cytokine Il17 expression was elevated in the skin of HSD mice. Expression of anti-inflammatory Il10 and Il13 decreased in the skin of HSD or HSD IMQ mice. The fibroblast marker Acta2 and ECM component Fn and Col1a1 decreased in HSD IMQ mice. Expression of ECM remodeling related Pdgfb was lower in HSD and HSD IMQ mice. In PBMCs, production of IL10, IL13 and PDGFB was reduced due to high salt loading. In cultured DFs high salt concentration resulted in decreased cell motility and ECM production, as well.
Conclusion: Our results demonstrate that high dietary salt intake is associated with increased dermal pro-inflammatory status. Interestingly, although inflammation induces the synthesis of ECM in most organs, the expression of ECM decreased in the inflamed skin of mice on high salt diet. Our data suggest that salt intake may alter the process of skin remodeling.
Funding: This work was supported by grant K125470, EFOP-3.6.3-VEKOP-16-2017-00009, 20382-3/2018 FEKUTSTRAT, 2020-4.1.1-TKP2020, and STIA-KFI-2020.
Semmelweis University, Károly Rácz Doctoral School of Clinical Medicine