PhD Scientific Days 2021

Budapest, 7-8 July 2021

PH_I_P: Pharmaceutical Sciences I. Posters

Formulation Of Nanofibrous Patches For The Treatment Of Herpes Labialis

Adrienn Kazsoki1, Gábor Katona2, Alán Alpár3, Romána Zelkó1

1University Pharmacy Department of Pharmacy Administration, Semmelweis University, Budapest
2University of Szeged, Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, Szeged
3Department of Anatomy, Semmelweis University, Budapest

Text of the abstract

The herpes labialis is caused by the herpes simplex virus type 1. Topically applied antiviral creams and patches are the commercially available products for the treatment. The patch seals the wound and arranges ideal conditions for wound healing and protects from the bacteria over-infections. Patches can be formed by nanofibrous layer, which can efficiently absorb exudates and facilitate cell respiration The drug-loaded nanofibrous mats offer a promising solution to the treatment of the herpes labialis.
The aim of the project was to advance the current state-of-the-art of preparing core-shell nanofibers using water-soluble polymers. To promote the process of the regeneration, core-shell type nanofibrous scaffolds loaded with dexpanthenol (shell) and acyclovir (core) were used. The further aim was to study the prepared electrospun scaffolds' morphological, structural, and physicochemical properties.
The fibrous samples were prepared by electrospinning (SpinSplit Ltd.) using water-soluble polymers; polyvinylpyrrolidone (PVP) as a shell, hypromellose (HPMC) and poly(ethylene oxide)(PEO) composite or poly(vinyl alcohol) (PVA) as a core polymer. The morphology structure of the prepared sample was studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Solid-state characterization was carried out by Raman spectroscopy.
The SEM photos showed that fibrous structures were obtained. In the case of the PVA/PVP core/shell fibers, the desired structure was obtained. while when HPMC-PEO were used as core, the core-shell structure could not be observed on the TEM photos. The Raman measurements revealed the mixed fiber structure of this sample, indicating that the interfacial stability between the inner and outer solution was not perfect. Raman spectroscopy measurements pointed out that amorphous solid dispersion was formed and homogenous drug distribution was obtained in the case of both fibrous sheets.
Coaxially electrospun fibers of different compositions were successfully prepared with various structural homogeneities. As homogenous drug distribution was obtained, both of the formulations could be suitable for the fibrous layer of the patches, and thus for the treatment of herpes labialis.
This project was supported by ÚNKP-20-4-I New National Excellence Program of the Ministry for Innovation and Technology.

University and Doctoral School

Semmelweis University, Doctoral School of Pharmaceutical Sciences